منابع مشابه
Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation
Early detection and proper management of kidney rejection are crucial for the long-term health of a transplant recipient. Recipients are normally monitored by serum creatinine measurement and sometimes with graft biopsies. Donor-derived cell-free deoxyribonucleic acid (cfDNA) in the recipient's plasma and/or urine may be a better indicator of acute rejection. We evaluated digital PCR (dPCR) as ...
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The purpose of research is focused on the insight into the future of Bitcoin on the financial situation, its implications and challenges. The problem of study is to investigate how to deal with a new type of digital currencies (such Bitcoin) that does not have a physical presence and there is no specific body to issue. Thus, this study aims to identify the nature of Bitcoin currency and what ar...
متن کاملDigital PCR.
The identification of predefined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. Here, we describe an approach for transforming the exponential, analog nature of the PCR into a linear, digital signal suitable for this purpose. Single molecules are isolated by dilution and individually amplified by ...
متن کاملDigital PCR
We are very pleased to be able to bring you this special issue of iomolecular Detection and Quantification which focuses on digital CR (dPCR). The underpinning method of dPCR, which was coined n 1999 [1], actually predates qPCR [2] and is a powerful technique hat could offer improved sensitivity, precision and reproducibility 3]. Now is an exciting time for this method and there are numerous xa...
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This paper describes a SlipChip to perform digital PCR in a very simple and inexpensive format. The fluidic path for introducing the sample combined with the PCR mixture was formed using elongated wells in the two plates of the SlipChip designed to overlap during sample loading. This fluidic path was broken up by simple slipping of the two plates that removed the overlap among wells and brought...
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ژورنال
عنوان ژورنال: Biomolecular Detection and Quantification
سال: 2016
ISSN: 2214-7535
DOI: 10.1016/j.bdq.2016.10.003